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KMID : 1134820000290061075
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 6 p.1075 ~ p.1082
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Effect of Crab Shell Supplementation in Feeds on Taurine Content of Cow’s Milk
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Park Dong-Jin
Lee Jee-Sun
Kim Dong-Shin
Park Tae-Sun
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Abstract
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Effects of dietary crab shell supplementation on milk taurine concentration were evaluated in 25 dairy cows fed one of the following feeds for 30 days : control feed, 1% crab shell meal supplemented feed (1%CS), 3% crab shell meal supplemented feed (3%CS), 5% crab shell meal supplemented feed (5%CS), or 3% formaldehyde treated crab shell meal supplemented feed (3%FCS). The crab shell meal used in this study consisted of the wasted parts of shells, excluding the front leg and back shells of snow crab (Chionoecetes japonicus). Amino acid concentrations of the crab shell and milk samples, either as a free form or a protein component, were determined using an automatic amino acid analyzer based on ion exchange chromatography. Analysis of the amino acid composition of crab shell protein hydro- lyzates showed that phenylalanine existed at the highest concentration, which was followed by glutamate, aspartate, glycine, serine, histidine and arginine. Unlike to the amino acid composition of most proteins found in animal tissues, branched-chain amino acid existed as relatively low levels in crab shell meal. Taurine was the most abundant free amino acid found in three different parts of the crab shell. Front leg shells contained 37% and 41% higher levels of taurine (509 μmol/100 g) compared to the back shell (319 μmol/100 g) and the crab shell meal (296 μmol/100 g), respectively. Effect of crab shell feeding on milk taurine concentration was significant at p<0.01 by ANOVA test. Cows fed 1%CS and 3%CS showed 20% and 49% increased milk taurine concentrations respectively, compared to the value for the control cows (p<0.01). Formaldehyde treatment of the crab shell meal was not effective in increasing the milk taurine concentration. This study is the first report suggesting the natural enhancement of taurine in cow’s milk by supplementing feeds with crab shell, and our results could be applied to the enhancement of taurine levels in other animal products.
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KEYWORD
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cow s milk, taurine, carb shell, amino acid composition, milk protein
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